miR-145-5p suppresses cell proliferation by targeting <i>IGF1R</i> and <i>NRAS</i> genes in multiple myeloma cells

Abstract Objectives Multiple myeloma (MM) is a common hematological cancer. Hence, it important to conduct further studies investigating the molecular mechanisms in detail that contributes genesis. In addition genetic changes, epigenetic factors such as miRNAs may influence expression of myeloma-related genes. Methods Our study aimed detect genes closely related MM and involved cancer process by changing these with bioinformatics tools vitro methods. Bioinformatics approaches identified hub our have role change connected myeloma. The functional impacts chosen miRNA on RPMI8226 U266 cell lines effect this changes putative target were investigated. Results viability miR-145-5p transfected cells was found decrease compared control IGF1R NRAS be significantly suppressed both at mRNA level. Decreased levels confirmed protein level well cells. addition, /miR-145-5p interaction demonstrated via luciferase reporter assay. However, EGFR , KLF4 IRS1 CDK4 CDK6 candidate had no statistically significant difference Conclusions Mir-145-5p act tumor suppressor inhibit proliferation targeting .